SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Step 1. The dual monochromator optics allow the widest. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. B: Selection of Excitation Wavelength. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Linear, Orbital Slow, and Orbital Fast. Between steps, reactions were rinsed 3 times with TBS + 0. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. 95. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). com. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 14 Average %CV 0. Our absorbance plate readers feature our PathCheck Sensor technology and. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. SDS-PAGE Assay. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. We would like to show you a description here but the site won’t allow us. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Molecular Devices. Molecular Devices Spectramax M5 Microplate Reader; Related products. 2. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Depending on which. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The following document is intended to demonstrate setup of this instrument. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Plate chamber design of the SpectraMax category 1 microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Figure 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. All associated StakMax® stackers. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Spectramax M5 microplate reader: Molecular Devices: N/A:. Amino acids assay. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. When a FlexStation® 3 Multi-Mode Microplate Reader. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. It cannot be used for luminescence measurements. Figure 1. 0 and 0. (The i3x and Paradigm readers require the. No filter change with dual monochromator optics. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Microplate Readers include the M3, M4, M5, and M5e models. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. 2. Uncontrolled Copy for Training and Reference Purposes Only . AquaMax® 4000. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. We use Spectramax M5 microplate reader in our lab. The spectrophotometric. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. SpectraMax® M5e. Microplate reader settings. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. A: Microplate Preparation. The cells were harvested on ice and. Add to Cart. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 72 MB. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax® iD3 Multi Mode Microplate . upgradeable with a wide range of high performance capabilities ideal for life science research and drug. Microplate Readers. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. STAKMAX. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. 1. The cuvette must be in place when you read the plate. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Choose from a. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Fill a clean plate with water. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 1 2. Download PDF. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Levels of immunoreactivity were normalized to. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Microplate Readers include the M3, M4,. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Wash Buffer Concentrate was diluted 10-fold. SpectraMax M5 Multi-Mode Microplate Reader. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 0 μL) instead of the sample solution was applied as control group, and. Table 1. microplate reader. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 0 out of. The raw data from each individual experiment were normalized to vehicle-treated HK-2. SpectraMax® iD5. Add to Cart. Introduction. Equipped with scanning, shaking and kinetics modules. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 5. The spectrophotometric. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Add to Cart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Spectramax Multi-Mode Microplate Reader Performance Specifications. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Figure 1. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax® M5. Sale price $18,499. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Turn on the microplate reader. Bioz Stars score: 86/100, based on 1 PubMed citations. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. Learn how you can utilize microplate readers to drive landmark discoveries. Learn how to set up protocols, customize reports, export results, and more with this PDF document. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax M5 reader settings are shown. The assay window spanned four orders of magnitude. The system. both cuvette-based and microplate reader applications on the same instrument. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. Fluorescence Microplate Reader Comparison Chart. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax iD5 Multi-Mode Microplate Reader. Example: [Drawer]. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The assay window spanned four orders of magnitude. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. view more . SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. When a feature or capability applies to only certain readers, this exception is noted. No additional manuals are available. 0 out of 5 $ 17,999 $ 16,995. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Molecular Devices. M3-M5e_manual. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Examples: “Press carefully to engage,” “ Do not. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Lift up the lid over the chamber to insert or remove a cuvette. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. 2. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. FlexStation™ III. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. The drawing shows a standard microplate of 15 mm height covered with a lid. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The following document is. The SpectraMax M2 and M2e are versatile and reliable. 1 Version 7. 0 2. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. These robust readers have been placed in labs from Antarctica to. Fluorescence Microplate Reader Comparison Chart. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. , USA). or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. book Page 7 Wednesday, July 7, 2010 2:09 PM. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. In . By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. ,. 0 . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 7°C (gradient heating design). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. Parallel negative control assays had primary, secondary, or both antibodies omitted. 7°C (gradient heating design). and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Supplier: Molecular Devices. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Turn on the microplate reader. Five preferred detection modes. 54 1. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. Keywords. ® reader to the computer. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. View the Molecular Devices website. Microplate Readers are modular and . Wide range of applications. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Bookings are free, but you will need to use iLab to book. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. AlphaScreen. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Qualify the luminescence performance of the SpectraMax . The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. . 4 Max StDev 1. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. ) by nonlinear regression using. 6. es •. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax® Microplate Readers and SoftMax® Pro Software. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. The SpectraMax M5e Multi. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. 2 only). Molecular Devices SpectraMax® series microplate readers. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. l. Most papers they cultured bacteria to OD600>0. TNF standard curve. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 67. 7. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Italic and boldface type are used for emphasis. 0. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. 00 USD. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Cuvette port functionality can also be controlled using SoftMax Pro Software. 6 0. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). 1 & 7. Temperature control range from Ambient +4º to 50ºC. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Access this by going to Help >. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 3. 0. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Key applications: ELISA, nucleic acid, endotoxin testing. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 4. Wide range of applications. We would like to show you a description here but the site won’t allow us. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). 95. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Call 516-939-0888 to check. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Compact and tunable absorbance microplate readers DATA SHEET. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 1** Version 7. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. The assay applies fluorescence polarization to quantify Fc-containing IgG. Go to Product Details. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2. To block unwanted residual excitation light and minimize background interference, a 570 nm. Wide range of applications The VersaMax reader covers. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Molecular Devices | Life Science Applications | Bioanalytical. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. (Shanghai, China). 0 out of 5 $ 2,499 $ 1,999. For the Live/Dead assay, they were treated. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Anne T. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). 7 cells were co-transfected with 0. Molecular Devices Spectramax M5 Microplate Reader. Add to Cart. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. Microplate Readers include the M3, M4, M5, and M5e models. Western blotting assays were conducted according to previous studies . Published Dec 16, 2020 | Updated Dec 16, 2020. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. E Models. Make sure the microplate is flat against. 3 Version 7. The PBS solution (10. Microplate Readers Include the M3, M4, M5, and M5. IMAP assays are based on binding of. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. The cells were then treated to induce apoptosis. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 306 plate air cal. Figure 4: Transcreener ADP. Data are normalized to vehicle-treated. TR-FRET assay principle. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate.